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Yao Yao, Rui Zhou, Rui Bai, Jing Wang, Mengjiao Tu, Jingjing Shi, Xiao He, Jinyun Zhou, Liu Feng, Yuanxue Gao, Fahuan Song, Feng Lan, Xingguo Liu, Mei Tian, Hong Zhang
《医学前沿(英文)》 2021年 第15卷 第3期 页码 472-485 doi: 10.1007/s11684-021-0832-y
关键词: neuronal progenitor cells resveratrol cerebral ischemia neuronal differentiation mitochondrial metabolism positron emission tomography
EFFECTS OF TRANSPORT STRESS ON THE INTESTINES INVOLVING NEURONAL NITRIC OXIDE SYNTHASE
《农业科学与工程前沿(英文)》 页码 285-295 doi: 10.15302/J-FASE-2022469
● Transport stress declined the level of leukocytes including lymphocytes in rat serum.
Protein aggregation in association with delayed neuronal death in rat model of brain ischemia
GE Pengfei, FU Shuanglin, LI Wenchen, WANG Chonghao, ZHOU Chuibing, LUO Yinan, LUO Tianfei
《医学前沿(英文)》 2008年 第2卷 第1期 页码 70-74 doi: 10.1007/s11684-008-0013-2
Construction of an immortalized neural progenitor cell strain and analysis of its immunogenicity
AN Ke, XU Ying, TIAN Xuebi, GAO Feng, TIAN Yuke, YANG Hui, ZHANG Chuanhan
《医学前沿(英文)》 2008年 第2卷 第1期 页码 63-69 doi: 10.1007/s11684-008-0012-3
关键词: immortalized long-term survival differentiation capacity genotype
Porcine pluripotent stem cells: progress, challenges and prospects
Jianyong HAN, Yi-Liang MIAO, Jinlian HUA, Yan LI, Xue ZHANG, Jilong ZHOU, Na LI, Ying ZHANG, Jinying ZHANG, Zhonghua LIU
《农业科学与工程前沿(英文)》 2019年 第6卷 第1期 页码 8-27 doi: 10.15302/J-FASE-2018233
Pluripotent stem cells (PSCs) are characterized by their capacity for high self-renewal and multiple differentiation potential and include embryonic stem cells, embryonic germ cells and induced PSCs. PSCs provide a very suitable model for the studies of human diseases, drugs screening, regenerative medicine and developmental biology research. Pigs are considered as an ideal model for preclinical development of human xenotransplantation, therapeutic approaches and regenerative medicine because of their size and physiological similarity to humans. However, lack of knowledge about the derivation, characterization and pluripotency mechanisms of porcine PSCs hinders progress in these biotechnologies. In this review, we discuss the latest progress on porcine PSCs generation, evaluation criteria for pluripotency, the scientific and technical questions arising from these studies. We also introduce our perspectives on porcine PSC research, in the hope of providing new ideas for generating naive porcine PSCs and animal breeding.
关键词: embryonic germ cells embryonic stem cells induced pluripotent stem cells pigs pluripotent stem cells
Determination of telomerase activity in stem cells and non-stem cells of breast cancer
LI Zhi, HE Yanli, ZHANG Jiahua, ZHANG Jinghui, HUANG Tao
《医学前沿(英文)》 2007年 第1卷 第3期 页码 294-298 doi: 10.1007/s11684-007-0056-9
Innate and adaptive T cells in influenza disease
null
《医学前沿(英文)》 2018年 第12卷 第1期 页码 34-47 doi: 10.1007/s11684-017-0606-8
Influenza is a major global health problem, causing infections of the respiratory tract, often leading to acute pneumonia, life-threatening complications and even deaths. Over the last seven decades, vaccination strategies have been utilized to protect people from complications of influenza, especially groups at high risk of severe disease. While current vaccination regimens elicit strain-specific antibody responses, they fail to generate cross-protection against seasonal, pandemic and avian viruses. Moreover, vaccines designed to generate influenza-specific T-cell responses are yet to be optimized. During natural infection, viral replication is initially controlled by innate immunity before adaptive immune responses (T cells and antibody-producing B cells) achieve viral clearance and host recovery. Adaptive T and B cells maintain immunological memory and provide protection against subsequent infections with related influenza viruses. Recent studies also shed light on the role of innate T-cells (MAIT cells, gd T cells, and NKT cells) in controlling influenza and linking innate and adaptive immune mechanisms, thus making them attractive targets for vaccination strategies. We summarize the current knowledge on influenza-specific innate MAIT and gd T cells as well as adaptive CD8+ and CD4+ T cells, and discuss how these responses can be harnessed by novel vaccine strategies to elicit cross-protective immunity against different influenza strains and subtypes.
关键词: influenza innate T cells CD4+ and CD8+ T cells vaccination
Reprogramming of the pig primordial germ cells into pluripotent stem cells: a brief review
Qijing LEI, Qin PAN, Shuai YU, Na LI, Shulin CHEN, Kuldip SIDHU, Jinlian HUA
《农业科学与工程前沿(英文)》 2019年 第6卷 第1期 页码 28-32 doi: 10.15302/J-FASE-2018222
Primordial germ cells (PGCs) are regarded as unipotent cells that can produce only either spermatogonia or oocytes. However, PGCs can be converted into the pluripotent state by first dedifferentiation to embryonic germ cells and then by reprogramming to induce them to become pluripotent stem cells (iPSCs). These two stages can be completely implemented with mouse cells. However, authentic porcine iPSCs have not been established. Here, we discuss recent advances in the stem cell field for obtaining iPSCs from PGCs. This knowledge will provide some clues which will contribute to the regulation of reprogramming to pluripotency in farm species.
关键词: pig pluripotent stem cells primordial germ cells reprogramming
4-1BBL expressed by eukaryotic cells activates immune cells and suppresses the progression of murine
Hui QIU, Hui ZHANG, Zuohua FENG
《医学前沿(英文)》 2009年 第3卷 第1期 页码 20-25 doi: 10.1007/s11684-009-0006-9
关键词: 4-1BB ligand tumor immunotherapy tumor microenvironment
The past, present and future of bovine pluripotent stem cells: a brief overview
Xiuchun TIAN
《农业科学与工程前沿(英文)》 2019年 第6卷 第1期 页码 3-7 doi: 10.15302/J-FASE-2018247
Although the pursuit of bovine embryonic stem cells started more than 26 years ago for the purpose of gene-targeting, true pluripotent stem cells in this economically important species are still elusive. With the rapid advances in genome-editing and cloning using homologously recombined somatic cells, the need for pluripotent stem cells for precise genetic modification in any species became questionable. With the pig being the better model for human regenerative biology, the identification of the commonalities and uniqueness of the pluripotency circuitry across mammalian species may be the main objective for studying pluripotent stem cells in the bovine.
Comparison of human nasal epithelial cells grown as explant outgrowth cultures or dissociated tissue
null
《医学前沿(英文)》 2013年 第7卷 第4期 页码 486-491 doi: 10.1007/s11684-013-0287-x
The purpose of this study was to compare cell growth characteristics, ciliated cell differentiation, and function of human nasal epithelial cells established as explant outgrowth cultures or dissociated tissue cultures. Human nasal mucosa of the uncinate process was obtained by endoscopy and epithelial cell cultures were established by explant outgrowth or dissociated tissue culture methods. Epithelial cell growth characteristics were observed by inverted phase contrast microscopy. Ciliated cell differentiation was detected by β-tubulin IV and ZO-1 immunocytochemistry. Basal and ATP-stimulated ciliary beat frequency (CBF) was measured using a high-speed digital microscopic imaging system. Both the explant and dissociated tissue cultures established as monolayers with tight junctions and differentiated cell composition, with both types of cultures comprising ciliated and non-ciliated epithelial cells. Fibroblasts were also frequently found in explant cultures but rarely seen in dissociated tissue cultures. In both culture systems, the highest ciliated cell density appeared at 7th–10th culture day and declined with time, with the lifespan of ciliated cells ranging from 14 to 21 days. Overall, 10% of the cells in explant cultures and 20% of the cells in the dissociated tissue cultures were ciliated. These two cultures demonstrated similar ciliary beat frequency values at baseline (7.78±1.99 Hz and 7.91±2.52 Hz, respectively) and reacted equivalently following stimulation with 100 μM ATP. The results of this study indicate that both the explant outgrowth and dissociated tissue culture techniques are suitable for growing well-differentiated nasal ciliated and non-ciliated cells, which have growth characteristics and ciliary activity similar to those of nasal epithelial cells in vivo.
关键词: ciliated cells ciliary beat frequency dissociated tissue culture explant culture nasal epithelial cells
null
《医学前沿(英文)》 2013年 第7卷 第3期 页码 345-353 doi: 10.1007/s11684-013-0282-2
Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) possess various advantageous properties, including self-renewal, extended proliferation potential, multi-lineage differentiation potential and capacity for differentiating into sweat gland-like cells in certain conditions. However, little is known about the effect of clinical-grade culture conditions on these properties and on the differentiative potential of hUC-MSCs. In this study, we sought to investigate the properties of hUC-MSCs expanded with animal serum free culture media (ASFCM) in order to determine their potential for differentiation into sweat gland-like cells. We found that primary cultures of hUC-MSCs could be established with ASFCM. Moreover, cells cultured in ASFCM showed vigorous proliferation comparable to those of cells grown in classical culture conditions containing fetal bovine serum (FBS). Morphology of hUC-MSCs cultured in ASFCM was comparable to those of cells grown under classical culture conditions, and hUC-MSCs grown in both of the two culture conditions tested showed the typical antigen profile of MSCs—positive for CD29, CD44, CD90, and CD105, and negative for CD34 and CD45, as expected. Chromosomal aberration assay revealed that the cells were stable after long-term culture under both culture conditions. Like normal cultured MSCs, hUC-MSCs induced under ASFCM conditions exhibited expression of the same markers (CEA, CK14 and CK19) and developmental genes (EDA and EDAR) that are characteristic of normal sweat gland cells. Taken together, our findings indicate that the classical culture medium used to differentiate hUC-MSCs into sweat gland-like cells can be replaced safely by ASFCM for clinical purposes.
关键词: umbilical cord mesenchymal stem cells sweat gland preclinical
Jingya SHEN,Yuliang SUN,Liping HUANG,Jinhui YANG
《环境科学与工程前沿(英文)》 2015年 第9卷 第6期 页码 1084-1095 doi: 10.1007/s11783-015-0805-y
关键词: biocathode microbial electrolysis cell microbial fuel cell Cu(II) removal Co(II) removal
Highlights for special issue on “Large Animal Stem Cells”
Jianyong HAN
《农业科学与工程前沿(英文)》 2019年 第6卷 第1期 页码 1-2 doi: 10.15302/J-FASE-2019251
null
《医学前沿(英文)》 2012年 第6卷 第1期 页码 41-47 doi: 10.1007/s11684-012-0175-9
Umbilical cord mesenchymal stem cells (MSCs) are a unique, accessible, and non-controversial source of early stem cells that can be readily manipulated. As the most common pluripotent cell, bone marrow-derived MSCs display limitations with the progress of stem cell therapy. By contrast, umbilical cord-derived cells, which have plentiful resources, are more accessible. However, several uncertain aspects, such as the effect of donor selection or culture conditions, long-term therapeutic effects, product consistency, and potential tumorigenicity, are the bottleneck in this clinical therapy. MSCs are predicted to undergo an unprecedented development in clinical treatment when a generally acknowledged criterion emerges. In the current paper, we highlight the application of umbilical cord-derived MSCs in skin therapies based on our previous studies, as well as the achievements of our peers in this field. This paper focuses on the strategies, challenges, and potential of this novel therapy.
关键词: umbilical cord mesenchymal stem cells cutaneous regeneration
标题 作者 时间 类型 操作
Resveratrol promotes the survival and neuronal differentiation of hypoxia-conditioned neuronal progenitorcells in rats with cerebral ischemia
Yao Yao, Rui Zhou, Rui Bai, Jing Wang, Mengjiao Tu, Jingjing Shi, Xiao He, Jinyun Zhou, Liu Feng, Yuanxue Gao, Fahuan Song, Feng Lan, Xingguo Liu, Mei Tian, Hong Zhang
期刊论文
Protein aggregation in association with delayed neuronal death in rat model of brain ischemia
GE Pengfei, FU Shuanglin, LI Wenchen, WANG Chonghao, ZHOU Chuibing, LUO Yinan, LUO Tianfei
期刊论文
Construction of an immortalized neural progenitor cell strain and analysis of its immunogenicity
AN Ke, XU Ying, TIAN Xuebi, GAO Feng, TIAN Yuke, YANG Hui, ZHANG Chuanhan
期刊论文
Porcine pluripotent stem cells: progress, challenges and prospects
Jianyong HAN, Yi-Liang MIAO, Jinlian HUA, Yan LI, Xue ZHANG, Jilong ZHOU, Na LI, Ying ZHANG, Jinying ZHANG, Zhonghua LIU
期刊论文
Determination of telomerase activity in stem cells and non-stem cells of breast cancer
LI Zhi, HE Yanli, ZHANG Jiahua, ZHANG Jinghui, HUANG Tao
期刊论文
Reprogramming of the pig primordial germ cells into pluripotent stem cells: a brief review
Qijing LEI, Qin PAN, Shuai YU, Na LI, Shulin CHEN, Kuldip SIDHU, Jinlian HUA
期刊论文
4-1BBL expressed by eukaryotic cells activates immune cells and suppresses the progression of murine
Hui QIU, Hui ZHANG, Zuohua FENG
期刊论文
Comparison of human nasal epithelial cells grown as explant outgrowth cultures or dissociated tissue
null
期刊论文
Capacity of human umbilical cord-derived mesenchymal stem cells to differentiate into sweat gland-likecells: a preclinical study
null
期刊论文
Microbial electrolysis cells with biocathodes and driven by microbial fuel cells for simultaneous enhanced
Jingya SHEN,Yuliang SUN,Liping HUANG,Jinhui YANG
期刊论文